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Anticorps Recombinant de lapin anti-CENPF

CENPF Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242277G9

N° de cat : 84865-4-PBS

Synonymes

242277G9, CENF, CENP-F, PRO1779

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPF Recombinant antibody (84865-4-RR)

    IF Staining of HeLa using 84865-4-RR (same clone as 84865-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CENPF Recombinant antibody (84865-4-RR)

    IF Staining of A431 using 84865-4-RR (same clone as 84865-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84865-4-RR

    Affinity and Kinetic Characterization of 84865-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

Informations sur le produit

84865-4-PBS cible CENPF dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène CENPF Protéine recombinante Ag29665
Nom complet centromere protein F, 350/400ka (mitosin)
Masse moléculaire calculée 368 kDa
Numéro d’acquisition GenBankNM_016343
Symbole du gène CENPF
Identification du gène (NCBI) 1063
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

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