Anticorps Polyclonal de lapin anti-CENPF

• Phare
• Validé par KD/KO

CENPF Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-28568

Synonymes

PRO1779, CENP-F, CENF

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Galerie de données de validation

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  IF Staining of A549 using CL488-28568

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

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  IF Staining of HeLa using CL488-28568

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

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  FC experiment of A549 using CL488-28568

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CENPF Polyclonal antibody (CL488-28568)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules A549, cellules HeLa
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-28568 cible CENPF dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CENPF Protéine recombinante Ag29665
• Nom complet: centromere protein F, 350/400ka (mitosin)
• Masse moléculaire calculée: 368 kDa
• Poids moléculaire observé: 350 kDa
• Numéro d’acquisition GenBank: NM_016343
• Symbole du gène: CENPF
• Identification du gène (NCBI): 1063
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

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