Anticorps Polyclonal de lapin anti-CENPO

CENPO Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20611-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20611-1-AP (same clone as 20611-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20611-1-AP (same clone as 20611-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 20611-1-AP (same clone as 20611-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 20611-1-AP (same clone as 20611-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20611-1-PBS cible CENPO dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CENPO Protéine recombinante Ag14619
• Nom complet: centromere protein O
• Masse moléculaire calculée: 300 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC002870
• Symbole du gène: CENPO
• Identification du gène (NCBI): 79172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.

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