Anticorps Polyclonal de lapin anti-CEP135

CEP135 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 24428-1-AP

Synonymes

centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635

---

Galerie de données de validation

• 

  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MG U-87, cellules U2OS
• Résultats positifs en IF: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

24428-1-AP cible CEP135 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP135 Protéine recombinante Ag19122
• Nom complet: centrosomal protein 135kDa
• Masse moléculaire calculée: 1140 aa, 134 kDa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: BC136535
• Symbole du gène: CEP135
• Identification du gène (NCBI): 9662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human		Elife; WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.; Authors - Emmanuelle Steib
human	IF	Cells; Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.; Authors - Zhenzhen Chu
human	IF	Mol Biol Cell; The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.; Authors - Alexander J Stemm-Wolf
human	IF	Gene; A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF).; Authors - Yan-Wei Sha

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Elisa (Verified Customer) (01-25-2023)

RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

CEP135 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24428-1-AP)