Anticorps Polyclonal de lapin anti-CEP170/CEP170L

• Phare
• Validé par KD/KO

CEP170/CEP170L Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 18899-1-AP

Synonymes

KIAA0470, KIAA0470L, CEP170L, CEP170

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Galerie de données de validation

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  WB analysis using 18899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 18899-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18899-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18899-1-AP

  Immunofluorescent analysis of Hela cells, using CEP170L antibody 18899-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Informations sur le produit

18899-1-AP cible CEP170/CEP170L dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP170/CEP170L Protéine recombinante Ag13532
• Nom complet: centrosomal protein 170kDa-like
• Masse moléculaire calculée: 170 kDa
• Poids moléculaire observé: 170-200 kDa
• Numéro d’acquisition GenBank: BC014590
• Symbole du gène: CEP170
• Identification du gène (NCBI): 645455
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEP170, also named as FAM68A, KAB and KIAA0470, is a fork head-associated (FHA) domain protein. CEP170L is a part of CEP170. The gene CEP170 has some isoforms with MW 170-200 kDa, 160 kDa and some fragments 30-40 kDa, ~75 kDa.

Publications

Species	Application	Title
human	WB,IF,IP	Mol Cancer; CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma.; Authors - Chunyan Gu
human	WB,IP	Signal Transduct Target Ther; BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability.; Authors - Xiaozhu Tang ; KD Validated
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang
human	WB	Acta Pharm Sin B; NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma; Authors - Rongfang Wei

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