Anticorps Monoclonal anti-CEP250,C-NAP1

CEP250,C-NAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2G12D12

N° de cat : 66814-1-PBS

Synonymes

Cep250, CEP250/CNAP1, 250 kDa centrosomal protein, 2G12D12, C NAP1

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Galerie de données de validation

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  WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66814-1-PBS cible CEP250,C-NAP1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CEP250,C-NAP1 Protéine recombinante Ag5925
• Nom complet: centrosomal protein 250kDa
• Masse moléculaire calculée: 281 kDa
• Poids moléculaire observé: 280 kDa
• Numéro d’acquisition GenBank: BC001433
• Symbole du gène: CEP250/CNAP1
• Identification du gène (NCBI): 11190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

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