Anticorps Polyclonal de lapin anti-CEP290

• Phare
• Validé par KD/KO

CEP290 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22490-1-PBS

Synonymes

CT87, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, BBS14, Bardet-Biedl syndrome 14 protein

---

Galerie de données de validation

• 

  WB analysis of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP (CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

  IP result of anti-CEP290 (IP:22490-1-AP, 4ug; Detection:22490-1-AP 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22490-1-AP (same clone as 22490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22490-1-AP (same clone as 22490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 22490-1-AP (same clone as 22490-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CEP290 antibody (22490-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

22490-1-PBS cible CEP290 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP290 Protéine recombinante Ag17457
• Nom complet: centrosomal protein 290kDa
• Masse moléculaire calculée: 2479 aa, 290 kDa
• Poids moléculaire observé: 290 kDa
• Numéro d’acquisition GenBank: BC008641
• Symbole du gène: CEP290
• Identification du gène (NCBI): 80184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. Catalog#22490-1-AP can recognise both.

{{ptg:RelatedPrimaryAntibodies}}