Anticorps Monoclonal anti-CEP290

CEP290 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C10G2

N° de cat : 67716-1-PBS

Synonymes

3H11Ag, BBS14, Cancer/testis antigen 87, centrosomal protein 290kDa, Centrosomal protein of 290 kDa, CEP290, CT87, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2

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Galerie de données de validation

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  WB analysis using 67716-1-Ig (same clone as 67716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67716-1-Ig (CEP290 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67716-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67716-1-Ig (same clone as 67716-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67716-1-Ig (CEP290 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67716-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67716-1-PBS cible CEP290 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CEP290 Protéine recombinante Ag17457
• Nom complet: centrosomal protein 290kDa
• Masse moléculaire calculée: 2479 aa, 290 kDa
• Poids moléculaire observé: 290 kDa
• Numéro d’acquisition GenBank: BC008641
• Symbole du gène: CEP290
• Identification du gène (NCBI): 80184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt.

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