Anticorps Monoclonal anti-CEP55

• Phare
• Validé par KD/KO

CEP55 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D11F7

N° de cat : 67460-1-PBS

Synonymes

Up-regulated in colon cancer 6, Up regulated in colon cancer 6, Centrosomal protein of 55 kDa, centrosomal protein 55kDa, C10orf3

---

Galerie de données de validation

• 

  WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

  WB result of CEP55 antibody (67460-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP55 antibody (67460-1-Ig, Clone: 2D11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67460-1-Ig (same clone as 67460-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67460-1-PBS cible CEP55 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CEP55 Protéine recombinante Ag29995
• Nom complet: centrosomal protein 55kDa
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 47-55 kDa
• Numéro d’acquisition GenBank: BC008947
• Symbole du gène: CEP55
• Identification du gène (NCBI): 55165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

{{ptg:RelatedPrimaryAntibodies}}