Anticorps Polyclonal de lapin anti-CEP83

CEP83 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26013-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 26013-1-AP (same clone as 26013-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26013-1-AP (CEP83 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26013-1-PBS cible CEP83 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP83 Protéine recombinante Ag22826
• Nom complet: coiled-coil domain containing 41
• Masse moléculaire calculée: 693 aa, 82 kDa
• Poids moléculaire observé: 83 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC125087
• Symbole du gène: CEP83
• Identification du gène (NCBI): 51134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively.

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