Anticorps Polyclonal de lapin anti-CEP97

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22050-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HL-60 using 22050-1-AP (same clone as 22050-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22050-1-AP (same clone as 22050-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 22050-1-AP (same clone as 22050-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

  IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 22050-1-AP (same clone as 22050-1-PBS)

  Immunofluorescent analysis of MDCK cells using 22050-1-AP (CEP97 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22050-1-PBS cible CEP97 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP97 Protéine recombinante Ag16995
• Nom complet: centrosomal protein 97kDa
• Masse moléculaire calculée: 865 aa, 97 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC041085
• Symbole du gène: CEP97
• Identification du gène (NCBI): 79598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

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