Anticorps Polyclonal de lapin anti-CERK

CERK Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25731-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 25731-1-AP (same clone as 25731-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 25731-1-AP (same clone as 25731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 25731-1-AP (same clone as 25731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25731-1-PBS cible CERK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CERK Protéine recombinante Ag22774
• Nom complet: ceramide kinase
• Masse moléculaire calculée: 537 aa, 60 kDa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC126940
• Symbole du gène: CERK
• Identification du gène (NCBI): 64781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.

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