Anticorps Polyclonal de lapin anti-Complement factor B

Complement factor B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 10170-1-AP

Synonymes

CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD

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Galerie de données de validation

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  WB analysis using 10170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 10170-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 10170-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: plasma humain, sang humain, Sanguin humain
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10170-1-AP cible Complement factor B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Complement factor B Protéine recombinante Ag0226
• Nom complet: complement factor B
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 93-100 kDa, 60-69 kDa
• Numéro d’acquisition GenBank: BC007990
• Symbole du gène: Complement factor B
• Identification du gène (NCBI): 629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

Publications

Species	Application	Title
human,mouse	IF	Nat Commun; The classical pathway triggers pathogenic complement activation in membranous nephropathy; Authors - Larissa Seifert
human	WB	J Cell Physiol; IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.; Authors - Xinxuan Cheng
human	WB,IHC,IF	Cell Oncol (Dordr); Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.; Authors - Reiri Shimazaki
human	WB	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
human,mouse	WB	Nephrol Dial Transplant; Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation.; Authors - Ming Wu
	WB	Chem Res Toxicol; 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.; Authors - Mikhail Linetsky

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

mark (Verified Customer) (03-30-2020)

worked with colourmetric immunohistochemistry

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: E13.5 to E18.5 embryo

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