Anticorps Polyclonal de lapin anti-Cofilin

• Phare
• Validé par KD/KO

Cofilin Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 10960-1-AP

Synonymes

18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

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Galerie de données de validation

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  WB analysis using 10960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10960-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skin using 10960-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10960-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10960-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10960-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10960-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 10960-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10960-1-AP (Cofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 10960-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cofilin antibody (10960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules NCI-H1299, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu cutané humain
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cardiaque humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10960-1-AP cible Cofilin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cofilin Protéine recombinante Ag1399
• Nom complet: cofilin 1 (non-muscle)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18-22 kDa
• Numéro d’acquisition GenBank: BC012318
• Symbole du gène: CFL1
• Identification du gène (NCBI): 1072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Publications

Species	Application	Title
	WB,IF,IP	Nat Commun; N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization; Authors - Weiji Weng
human	WB,IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang ; KD Validated
human	WB	Int J Mol Sci; Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.; Authors - Marina Damato
mouse	WB	Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	IHC	Oncotarget; A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.; Authors - Hui-Hui Cao

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