Anticorps Monoclonal anti-CFTR

• Phare
• Validé par KD/KO

CFTR Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2F4A8

N° de cat : 66928-1-PBS

Synonymes

Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7

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Galerie de données de validation

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  WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

  WB result of CFTR antibody (66928-1-Ig; 1:4000; room temperature for 1.5 hours) with wild-type and CFTR knockout HEK-293T cells. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66928-1-PBS cible CFTR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CFTR Protéine recombinante Ag27810
• Nom complet: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• Masse moléculaire calculée: 168 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_000492
• Symbole du gène: CFTR
• Identification du gène (NCBI): 1080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.

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