Anticorps Polyclonal de lapin anti-CHAT

CHAT Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 24418-1-AP

Synonymes

CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2

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Galerie de données de validation

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  WB analysis of rat brain using 24418-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 24418-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24418-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 24418-1-AP

  IP Result of anti-CHAT (IP:24418-1-AP, 4ug; Detection:24418-1-AP 1:300) with rat brain tissue lysate 4000ug.

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  IHC staining of rat brain using 24418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 24418-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24418-1-AP (CHAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

24418-1-AP cible CHAT dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHAT Protéine recombinante Ag19550
• Nom complet: choline acetyltransferase
• Masse moléculaire calculée: 748 aa, 83 kDa
• Poids moléculaire observé: 60-66 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC130617
• Symbole du gène: CHAT
• Identification du gène (NCBI): 1103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively.

Publications

Species	Application	Title
mouse	IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	IF	bioRxiv; Expression of the ACE2 virus entry protein in the nervus terminalis reveals the potential for an alternative route to brain infection in COVID-19.; Authors - Katarzyna Bilinska
mouse	IF	Neurobiol Dis; NRF2 activation suppresses motor neuron ferroptosis induced by the SOD1G93A mutation and exerts neuroprotection in amyotrophic lateral sclerosis; Authors - Biying Yang