Anticorps Polyclonal de lapin anti-CHAT

CHAT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 24418-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 24418-1-AP (same clone as 24418-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IP experiment of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  IP result of anti-CHAT (IP:24418-1-AP, 4ug; Detection:24418-1-AP 1:2000) with rat brain tissue lysate 1880 ug. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24418-1-AP (CHAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

24418-1-PBS cible CHAT dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHAT Protéine recombinante Ag19550
• Nom complet: choline acetyltransferase
• Masse moléculaire calculée: 748 aa, 83 kDa
• Poids moléculaire observé: 60-66 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC130617
• Symbole du gène: CHAT
• Identification du gène (NCBI): 1103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively.

{{ptg:RelatedPrimaryAntibodies}}