Anticorps Polyclonal de lapin anti-CHCHD10

• Phare
• Validé par KD/KO

CHCHD10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 25671-1-AP

Synonymes

C22orf16, CHCHD10, N27C7 4, Protein N27C7 4

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Galerie de données de validation

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  WB analysis using 25671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25671-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 25671-1-AP

  HAP1 (WT and CHCHD10 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 25671-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 25671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25671-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HAP1 using 25671-1-AP

  HAP1 lysates prepared and IP of CHCHD10 performed using 1.0 μg of 25671-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of mouse heart using 25671-1-AP

  IP Result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:25671-1-AP 1:600) with mouse heart tissue lysate 4000ug.

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  IHC staining of human kidney using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25671-1-AP (CHCHD10 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25671-1-AP (CHCHD10 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 25671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HAP1 using 25671-1-AP

  HAP1 WT cells (yellow outline) and CHCHD10 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 25671-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IF Staining of HeLa using 25671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de souris
• Résultats positifs en IP: tissu cardiaque de souris, HAP1 cells
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, HAP1

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

25671-1-AP cible CHCHD10 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHCHD10 Protéine recombinante Ag22598
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 10
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC065232
• Symbole du gène: CHCHD10
• Identification du gène (NCBI): 400916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.; Authors - Jung-A A Woo ; KD Validated
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
mouse	IHC	J Mol Cell Biol; Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.; Authors - Jian Peng
mouse	WB	Diabetes; CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis; Authors - Meng Ding ; KO Validated
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
human	WB,IF	Hum Mol Genet; Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.; Authors - Isabella R Straub ; KD Validated