Anticorps Monoclonal anti-CHCHD2

• Phare
• Validé par KD/KO

CHCHD2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

CloneNo.

2G1G10

Avis (1)

N° de cat : 66302-1-Ig

Synonymes

2G1G10, AAG10, Aging-associated gene 10 protein, C7orf17, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2

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Galerie de données de validation

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  WB analysis using 66302-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 66302-1-Ig

  WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

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  WB analysis using 66302-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66302-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66302-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66302-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66302-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (66302-1-Ig, Clone: 2G1G10 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules L02, cellules LNCaP, cellules SMMC-7721, tissu hépatique de porc, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

66302-1-Ig cible CHCHD2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHCHD2 Protéine recombinante Ag24219
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 2
• Masse moléculaire calculée: 151 aa, 16 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC003079
• Symbole du gène: CHCHD2
• Identification du gène (NCBI): 51142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; A nucleolar long "non-coding" RNA encodes a novel protein that functions in response to stress; Authors - Shuang Feng
human	WB	Proc Natl Acad Sci U S A; Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.; Authors - Siddhesh Aras
human	WB,IHC,IF	Acta Neuropathol Commun; Reduced erythrocytic CHCHD2 mRNA is associated with brain pathology of Parkinson's disease.; Authors - Xiaodan Liu
human	WB	Hum Mol Genet; Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.; Authors - Isabella R Straub ; KD Validated
	WB,IF	Hum Mol Genet; Mouse midbrain dopaminergic neurons survive loss of the PD-associated mitochondrial protein CHCHD2.; Authors - Mai K Nguyen
human	WB	Hum Mol Genet; Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.; Authors - Yi-Ting Liu ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vignesh (Verified Customer) (09-19-2025)

The results are excellent, bands are clear and highly sensitive

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 231 cell line

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