Anticorps Recombinant de lapin anti-CHCHD3/MIC19

• Phare
• Validé par KD/KO

CHCHD3/MIC19 Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4B11

N° de cat : 82743-1-PBS

Synonymes

CHCHD3, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, MIC19, MICOS complex subunit MIC19, MINOS3

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Galerie de données de validation

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  WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

  WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 82743-1-RR (same clone as 82743-1-PBS)

  IP result of anti-CHCHD3 (IP:82743-1-RR, 4ug; Detection:82743-1-RR 1:1000) with Jurkat cells lysate 1600 ug. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82743-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

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Informations sur le produit

82743-1-PBS cible CHCHD3/MIC19 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHCHD3/MIC19 Protéine recombinante Ag22579
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 3
• Masse moléculaire calculée: 227 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC014839
• Symbole du gène: CHCHD3
• Identification du gène (NCBI): 54927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

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