Anticorps Monoclonal anti-CHCHD4

CHCHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E4C6

N° de cat : 66718-1-PBS

Synonymes

CHCHD4, FLJ31709, MIA40

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Galerie de données de validation

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  WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66718-1-Ig (same clone as 66718-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using CHCHD4 antibody (66718-1-Ig, Clone: 3E4C6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66718-1-PBS cible CHCHD4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHCHD4 Protéine recombinante Ag15339
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 4
• Masse moléculaire calculée: 142 aa, 16 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC033775
• Symbole du gène: CHCHD4
• Identification du gène (NCBI): 131474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

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