Anticorps Polyclonal de lapin anti-CHD2

• Phare
• Validé par KD/KO

CHD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21334-1-PBS

Synonymes

EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 2, CHD-2, CHD 2, ATP-dependent helicase CHD2

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Galerie de données de validation

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  WB analysis using 21334-1-AP (same clone as 21334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21334-1-AP (CHD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21334-1-AP (same clone as 21334-1-PBS)

  WB result of CHD2 antibody (21334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21334-1-AP (same clone as 21334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21334-1-AP (same clone as 21334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21334-1-PBS cible CHD2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHD2 Protéine recombinante Ag15429
• Nom complet: chromodomain helicase DNA binding protein 2
• Masse moléculaire calculée: 1828 aa, 211 kDa
• Poids moléculaire observé: 230-250 kDa
• Numéro d’acquisition GenBank: BC020810
• Symbole du gène: CHD2
• Identification du gène (NCBI): 1106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chromodomain helicase DNA binding protein 2 (CHD2), a member of the sucrose nonfermenting (SNF-2) protein family of epigenetic regulators, which use the energy from ATP hydrolysis to change nucleosome positioning, composition and chromatin structure(PMID: 33435571). CHD2 protein plays a critical role in embryonic development, tumor suppression and survival(PMID: 24834135). In addition, CHD2 has been proposed to prevent breast cancer initiation, and CHD2 mutations are associated with chronic lymphocytic leukemia.

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