Anticorps Polyclonal de lapin anti-CHD7

CHD7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31919-1-PBS

Synonymes

ATP-dependent helicase CHD7, CHD-7, Chromodomain Helicase DNA Binding Protein 7, Chromodomain-helicase-DNA-binding protein 7, EC:3.6.4.12

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Galerie de données de validation

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  WB analysis using 31919-1-AP (same clone as 31919-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 31919-1-AP (CHD7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 31919-1-AP (same clone as 31919-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CHD7 antibody (31919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31919-1-PBS cible CHD7 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHD7 Protéine recombinante Ag36595
• Nom complet: chromodomain helicase DNA binding protein 7
• Poids moléculaire observé: 350 kDa
• Numéro d’acquisition GenBank: BC110818
• Symbole du gène: CHD7
• Identification du gène (NCBI): 55636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent eukaryotic chromatin remodeling enzyme that regulates nucleosome positioning and alters DNA accessibility, and is essential for organ development.CHD7 is a gene known to be associated with CHARGE syndrome, Kallmann syndrome, and hypogonadotropic hypogonadism, where it is associated with CHARGE syndrome is a congenital multiorgan disorder characterized by eye defects, heart defects, posterior nasal atresia, growth retardation, genital anomalies, ear malformations, and deafness. The effects of CHD7 mutations on inner ear development, neuronal differentiation, cardiovascular development, and regulation of bone lipid homeostasis have been studied.

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