Anticorps Recombinant de lapin anti-CHD8

CHD8 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250612C4

N° de cat : 86032-1-RR

Synonymes

CHD 8, HELSNF1

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Galerie de données de validation

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  WB analysis using 86032-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86032-1-RR (CHD8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 86032-1-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86032-1-RR (CHD8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86032-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86032-1-RR (CHD8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86032-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD8 antibody (86032-1-RR, Clone: 250612C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86032-1-RR cible CHD8 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHD8 Protéine recombinante Ag31425
• Nom complet: chromodomain helicase DNA binding protein 8
• Masse moléculaire calculée: 290KD
• Poids moléculaire observé: 290 kDa
• Numéro d’acquisition GenBank: NM_001170629
• Symbole du gène: CHD8
• Identification du gène (NCBI): 57680
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromodomain helicase domain 8 (CHD8) is one of the most frequently mutated and most penetrant genes in the autism spectrum disorder (ASD). CHD8 is located on 14q11.2. It is part of the SNF2H-like ATP-dependent chromatin remodeling enzymes family referred to as CHD (chromodomain helicase DNA binding). CHD8 has two isoforms: CHD8L, a full-length protein of 280 kDa; and CHD8S (Duplin), a 110 kDa protein of the NH2-terminal chromodomain region resulting from alternative splicing (PMID:19151705). CHD8 is essential for development, as homozygote mutant mice die at an embryonic stage. CHD8 is expressed in the mouse at the embryonic stage (E12.5) in different levels in wide regions of the brain (neocortex, forebrain, ventricular, subventricular and mantle zones, rhombic lip (RL), and the isthmus of the cerebellum, as well as in lower RL and floor plate region of the hindbrain, midbrain, diencephalon, hypothalamus, pituitary gland, craniofacial region, and tongue and olfactory epithelium). In the postnatal mouse brain (P20), CHD8 is expressed in the cerebellum, neocortex, hippocampus, hypothalamus, and olfactory bulb (PMID:30277262). Peak expression levels were observed at E18-P7, then gradually decreased to adulthood. Highest expression was found in neurons, and lower levels in astrocyte and astroglia (PMID:30574290). In the mouse, CHD8 is expressed higher in brain compared to other tissues and in the embryo, compared to adult.

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