Anticorps Polyclonal de lapin anti-CHD9

• Phare
• Validé par KD/KO

CHD9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13402-1-PBS

Synonymes

AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator

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Galerie de données de validation

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  WB analysis using 13402-1-AP (same clone as 13402-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 13402-1-AP (same clone as 13402-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

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  IF Staining of Hela using 13402-1-AP (same clone as 13402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using CHD9 antibody (13402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13402-1-PBS cible CHD9 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHD9 Protéine recombinante Ag4245
• Nom complet: chromodomain helicase DNA binding protein 9
• Masse moléculaire calculée: 2897 aa, 326 kDa
• Poids moléculaire observé: 330 kDa
• Numéro d’acquisition GenBank: BC033770
• Symbole du gène: CHD9
• Identification du gène (NCBI): 80205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).

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