Anticorps Recombinant de lapin anti-Chromogranin A

Chromogranin A Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243048H7

N° de cat : 85605-2-PBS

Synonymes

CGA, CHGA, AL-11, Chromogranin-A, EA-92

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Galerie de données de validation

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  WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

  Pig adrenal gland tissue was subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 85605-2-RR (same clone as 85605-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendix using 85605-2-RR (same clone as 85605-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 85605-2-RR (same clone as 85605-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 85605-2-RR (same clone as 85605-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 85605-2-RR (same clone as 85605-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 85605-2-RR (same clone as 85605-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 85605-2-RR (same clone as 85605-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 85605-2-RR (same clone as 85605-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85605-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85605-2-RR against Human Chromogranin A were performed. The affinity constant is 3.39 nM.

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Informations sur le produit

85605-2-PBS cible Chromogranin A dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Chromogranin A Protéine recombinante Ag19780
• Nom complet: chromogranin A (parathyroid secretory protein 1)
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC006459
• Symbole du gène: Chromogranin A
• Identification du gène (NCBI): 1113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

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