Anticorps Recombinant de lapin anti-CHGB

CHGB Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242952C9

N° de cat : 85438-4-PBS

Synonymes

CgB, Chromogranin B, SCG1, 242952C9, Chromogranin-B

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Galerie de données de validation

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  WB analysis using 85438-4-RR (same clone as 85438-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

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  WB analysis using 85438-4-RR (same clone as 85438-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 85438-4-RR (same clone as 85438-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85438-4-RR (CHGB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 85438-4-RR (same clone as 85438-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85438-4-RR (CHGB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85438-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85438-4-RR against Human CHGB were performed. The affinity constant is 1.95 nM.

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Informations sur le produit

85438-4-PBS cible CHGB dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHGB Protéine recombinante Ag6833
• Nom complet: chromogranin B (secretogranin 1)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70-100 kDa
• Numéro d’acquisition GenBank: BC000375
• Symbole du gène: Chromogranin B
• Identification du gène (NCBI): 1114
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa.

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