Anticorps Polyclonal de lapin anti-Chk1

• Phare
• Validé par KD/KO

Chk1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 10362-1-AP

Synonymes

CHEK1, Chk1

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Galerie de données de validation

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  WB analysis of mouse thymus using 10362-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10362-1-AP

  Immunofluorescent analysis of HepG2 cells using 10362-1-AP (Chk1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HepG2 using 10362-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CHK1 antibody (10362-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules HeLa, cellules K-562
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 1 publications below

Informations sur le produit

10362-1-AP cible Chk1 dans les applications de WB, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Chk1 Protéine recombinante Ag0409
• Nom complet: CHK1 checkpoint homolog (S. pombe)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC004202
• Symbole du gène: CHEK1
• Identification du gène (NCBI): 1111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated(PMID:22941630) and ubiquitinated (PMID:19276361). It has 3 isoforms produced by alternative splicing with the molecular weight of 54 kDa, 44 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons.; Authors - Andrew Best ; KD Validated
	WB	Proc Natl Acad Sci U S A; Checkpoint kinase 1 (Chk1)-short is a splice variant and endogenous inhibitor of Chk1 that regulates cell cycle and DNA damage checkpoints.; Authors - Navjotsingh Pabla
human	WB	Oncotarget; UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.; Authors - Xiaojia Gao
human	WB	EBioMedicine; High PARP-1 expression predicts poor survival in acute myeloid leukemia and PARP-1 inhibitor and SAHA-bendamustine hybrid inhibitor combination treatment synergistically enhances anti-tumor effects.; Authors - Xia Li
human	WB	Cell Death Dis; HBV infection potentiates resistance to S-phase arrest-inducing chemotherapeutics by inhibiting CHK2 pathway in diffuse large B-cell lymphoma.; Authors - Xinying Zhao
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi