Anticorps Polyclonal de lapin anti-Chk1

• Phare
• Validé par KD/KO

Chk1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(48)

Avis (1)

N° de cat : 25887-1-AP

Synonymes

CHEK1, EC:2.7.11.1, CHK1 checkpoint homolog, Chk 1, CHEK 1

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Galerie de données de validation

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  WB analysis using 25887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 25887-1-AP

  IP result of anti-Chk1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug.

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  IHC staining of human kidney using 25887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293T using 25887-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 25887-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293T
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293T,
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

25887-1-AP cible Chk1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Chk1 Protéine recombinante Ag22993
• Nom complet: CHK1 checkpoint homolog (S. pombe)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC004202
• Symbole du gène: Chk1
• Identification du gène (NCBI): 1111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability; Authors - Zaili Luo
human	WB	Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
human	WB	Nat Commun; G-quadruplex DNA structures in human stem cells and differentiation.; Authors - Katherine G Zyner
human	WB	Cell Death Differ; Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.; Authors - Yan Zhao
human	WB	Cancer Res; Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.; Authors - Zhe Sun
human	WB	Clin Cancer Res; RNF126 as a biomarker of a poor prognosis in invasive breast cancer and CHK1 inhibitor efficacy in breast cancer cells.; Authors - Xiaosong Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

k. (Verified Customer) (10-26-2023)

The antibody worked for WB at a 1:100 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

Chk1 Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:25887-1-AP)

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