Anticorps Polyclonal de lapin anti-CHKA-Specific

CHKA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 19537-1-AP

Synonymes

CHETK alpha, CHK, CHKA, CHKA-Specific, Choline kinase alpha, CK, CKI, EK, Ethanolamine kinase;

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Galerie de données de validation

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  WB analysis of HepG2 using 19537-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19537-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 19537-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19537-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19537-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human breast cancer using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human liver using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 19537-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 19537-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHKA antibody 19537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules Raji, tissu de côlon de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19537-1-AP cible CHKA-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: choline kinase alpha
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: NM_001277
• Symbole du gène: CHKA
• Identification du gène (NCBI): 1119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.

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