Anticorps Polyclonal de lapin anti-CHMP2A

• Phare
• Validé par KD/KO

CHMP2A Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10477-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 10477-1-AP (same clone as 10477-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10477-1-AP (same clone as 10477-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10477-1-AP (same clone as 10477-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10477-1-PBS cible CHMP2A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHMP2A Protéine recombinante Ag0719
• Nom complet: chromatin modifying protein 2A
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002502
• Symbole du gène: CHMP2A
• Identification du gène (NCBI): 27243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in the degradation of surface receptor proteins and the formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).

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