Anticorps Monoclonal anti-CHMP4B

CHMP4B Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D4G10

N° de cat : 68811-1-Ig

Synonymes

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

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Galerie de données de validation

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  WB analysis using 68811-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68811-1-Ig (CHMP4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HCT 116 using 68811-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using CHMP4B antibody (68811-1-Ig, Clone: 1D4G10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MOLT-4
• Résultats positifs en IF/ICC: cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68811-1-Ig cible CHMP4B dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHMP4B Protéine recombinante Ag4544
• Nom complet: chromatin modifying protein 4B
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC033859
• Symbole du gène: CHMP4B
• Identification du gène (NCBI): 128866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

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