Anticorps Monoclonal anti-CHOP; GADD153

CHOP; GADD153 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

CloneNo.

4F3G1

N° de cat : 66741-1-Ig

Synonymes

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153

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Galerie de données de validation

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  WB analysis using 66741-1-Ig

  Un-treated and Tunicamycin treated HepG2 lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66741-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66741-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66741-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66741-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66741-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules C6, cellules HepG2, cellules HepG2 traitées à la tunicamycine, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

66741-1-Ig cible CHOP; GADD153 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHOP; GADD153 Protéine recombinante Ag7354
• Nom complet: DNA-damage-inducible transcript 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003637
• Symbole du gène: DDIT3
• Identification du gène (NCBI): 1649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publications

Species	Application	Title
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto
human,mouse	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
mouse	WB	Front Immunol; miR-10a-3p modulates adiposity and suppresses adipose inflammation through TGF-β1/Smad3 signaling pathway; Authors - Sonia Kiran
human	WB	Biochem Pharmacol; 2-Deoxy-D-glucose increases the sensitivity of glioblastoma cells to BCNU through the regulation of glycolysis, ROS and ERS pathways: In vitro and in vivo validation.; Authors - Xiaodong Sun
human	WB	Ecotoxicol Environ Saf; The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.; Authors - Jing Wu
rat	WB	Ecotoxicol Environ Saf; Role of endoplasmic reticulum stress in cadmium-induced hepatocyte apoptosis and the protective effect of quercetin.; Authors - Jicang Wang