Anticorps Monoclonal anti-CHOP/GADD153

CHOP/GADD153 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F3G1

N° de cat : 66741-1-PBS

Synonymes

CHOP; GADD153, CHOP, DDIT3, GADD153, 4F3G1

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Galerie de données de validation

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  WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

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  WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

  Un-treated and Tunicamycin treated HepG2 lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66741-1-Ig (same clone as 66741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66741-1-Ig (same clone as 66741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66741-1-PBS cible CHOP/GADD153 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHOP/GADD153 Protéine recombinante Ag7354
• Nom complet: DNA-damage-inducible transcript 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003637
• Symbole du gène: CHOP
• Identification du gène (NCBI): 1649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

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