Anticorps Recombinant de lapin anti-CHOP/GADD153

CHOP/GADD153 Recombinant Antibody for WB, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

1G11

N° de cat : 81462-1-RR

Synonymes

CHOP; GADD153, CHOP, DDIT3, GADD153, 1G11

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Galerie de données de validation

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  WB analysis of HeLa using 81462-1-RR

  Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 81462-1-RR

  Untreated and tunicamycin treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  ChIP experiment of NIH/3T3 using 81462-1-RR

  Chromatin was prepared from NIH/3T3 cells treated with Tunicamycin (2ug/ml, overnigh). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of CHOP (81462-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

• 

  Affinity and Kinetic Characterization of 81462-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la tunicamycine, Tunicamycin treated Jurkat cells
• Résultats positifs en ChIP-qPCR: Tunicamycin (2ug/ml, overnigh) NIH/3T3 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81462-1-RR cible CHOP/GADD153 dans les applications de WB, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHOP/GADD153 Protéine recombinante Ag7354
• Nom complet: DNA-damage-inducible transcript 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003637
• Symbole du gène: CHOP
• Identification du gène (NCBI): 1649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

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