Anticorps Polyclonal de lapin anti-CHRNA7/CHRFAM7A

• Phare
• Validé par KD/KO

CHRNA7/CHRFAM7A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 21586-1-AP

Synonymes

CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10

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Galerie de données de validation

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  WB analysis of Jurkat using 21586-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21586-1-AP

  WB result of CHRNA7/CHRFAM7A antibody (21586-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7/CHRFAM7A transfected Jurkat cells.

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  WB analysis of Jurkat using 21586-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21586-1-AP cible CHRNA7/CHRFAM7A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHRNA7/CHRFAM7A Protéine recombinante Ag16151
• Nom complet: CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC101348
• Symbole du gène: CHRFAM7A
• Identification du gène (NCBI): 89832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuronal acetylcholine receptor subunit alpha-7, encoded by the CHRNA7 gene, is involved in cognition through interneuron modulation of 4-(2-aminoethyl)benzene-1,2-diol and glutamate signaling. CHRNA7 gene is genetically linked to multiple disorders with cognitive deficits. A partial duplication of CHRNA7 (CHRFAM7A) is found in humans. CHRFAM7A gene encodes the CHRNA7-FAM7A fusion protein. Expression of CHRFAM7A has been reported in brain, peripheral blood lymphocytes (PBLs), and a broad range of epithelial cells (PMID: 21718690; 23553139; 25681457). CHRFAM7A has been associated with many neuropsychiatric disorders (PMID: 25906356; 25701707). This antibody, raised against 241-386 amino acids of human CHRFAM7A protein, recognizes both CHRNA7 and CHRFAM7A.