Anticorps Polyclonal de lapin anti-CHRNA3

• Phare
• Validé par KD/KO

CHRNA3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10333-1-PBS

Synonymes

Neuronal acetylcholine receptor subunit alpha-3, NACHRA3

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Galerie de données de validation

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  WB analysis using 10333-1-AP (same clone as 10333-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 10333-1-AP (same clone as 10333-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10333-1-AP (same clone as 10333-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10333-1-AP (same clone as 10333-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10333-1-AP (CHRNA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10333-1-PBS cible CHRNA3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHRNA3 Protéine recombinante Ag0272
• Nom complet: cholinergic receptor, nicotinic, alpha 3
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000513
• Symbole du gène: CHRNA3
• Identification du gène (NCBI): 1136
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.

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