Anticorps Recombinant de lapin anti-CHRNA7

CHRNA7 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240117D12

N° de cat : 82848-4-PBS

Synonymes

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12

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Galerie de données de validation

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  WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

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  WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 82848-4-RR (same clone as 82848-4-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 82848-4-RR (same clone as 82848-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82848-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82848-4-PBS cible CHRNA7 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHRNA7 Protéine recombinante Ag15980
• Nom complet: cholinergic receptor, nicotinic, alpha 7
• Masse moléculaire calculée: 502 aa, 56 kDa
• Poids moléculaire observé: 50 kDa, 56 kDa
• Numéro d’acquisition GenBank: BC037571
• Symbole du gène: CHRNA7
• Identification du gène (NCBI): 1139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

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