Anticorps Polyclonal de lapin anti-CHRNB4

• Phare
• Validé par KD/KO

CHRNB4 Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 22192-1-AP

Synonymes

CHRNB4

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 22192-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22192-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 22192-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNB4 antibody (22192-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HeLa, tissu testiculaire de souris
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

22192-1-AP cible CHRNB4 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHRNB4 Protéine recombinante Ag16767
• Nom complet: cholinergic receptor, nicotinic, beta 4
• Masse moléculaire calculée: 498 aa, 56 kDa
• Poids moléculaire observé: 48-60 kDa
• Numéro d’acquisition GenBank: BC096080
• Symbole du gène: CHRNB4
• Identification du gène (NCBI): 1143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	FC	Mar Drugs; Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.; Authors - Zhihua Sun
human	WB	Eur J Pharmacol; Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth.; Authors - Jiang Qian
human	WB	Cancers (Basel); An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer.; Authors - Yi-Hsuan Chuang ; KO Validated
human	WB	Mar Drugs; Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.; Authors - Yiqiao Liu