Anticorps Polyclonal de lapin anti-CHST3

CHST3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 18242-1-AP

Synonymes

C6ST, C6ST 1, C6ST1, Chondroitin 6 sulfotransferase, CHST3, GST 0, HSD

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Galerie de données de validation

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  WB analysis of mouse heart using 18242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18242-1-AP (CHST3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18242-1-AP (CHST3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18242-1-AP (CHST3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18242-1-AP (CHST3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

18242-1-AP cible CHST3 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHST3 Protéine recombinante Ag13008
• Nom complet: carbohydrate (chondroitin 6) sulfotransferase 3
• Masse moléculaire calculée: 479 aa, 55 kDa
• Poids moléculaire observé: 55-67 kDa
• Numéro d’acquisition GenBank: BC093690
• Symbole du gène: CHST3
• Identification du gène (NCBI): 9469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.; Authors - Laura Hobohm
human	IHC	Osteoarthritis Cartilage; Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease.; Authors - Jing Han
mouse	IF	Cells; Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.; Authors - David Baidoe-Ansah
mouse	IHC	J Neurochem; OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex.; Authors - Hiroaki Okuda
human	IHC	Histochem Cell Biol; Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.; Authors - Jian Lei
human	WB	Biol Trace Elem Res; Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes.; Authors - Liyun Wang

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