Anticorps Monoclonal anti-CHST4

• Phare
• Validé par KD/KO

CHST4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1E1A4

Avis (1)

N° de cat : 66623-1-Ig

Synonymes

Gn6st-2, Gn6st 2, GlcNAc6ST-2, GlcNAc6ST 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3

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Galerie de données de validation

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  WB analysis of HT-29 using 66623-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66623-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66623-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66623-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66623-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66623-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66623-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CHST4 antibody (66623-1-Ig, Clone: 1E1A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 66623-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66623-1-Ig (CHST4 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules COLO 320, cellules MCF-7, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

66623-1-Ig cible CHST4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHST4 Protéine recombinante Ag3987
• Nom complet: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
• Masse moléculaire calculée: 370 aa, 43 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC035282
• Symbole du gène: CHST4
• Identification du gène (NCBI): 10164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)

Publications

Species	Application	Title
human	IHC	Genomics Proteomics Bioinformatics; miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.; Authors - Bo Hu
mouse,human	WB,IHC	Front Oncol; Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.; Authors - Longshan Zhang ; KD Validated
human	IHC	J Leukoc Biol; CHST4 associates with high-abundance immune infiltration in hormone receptor-positive breast cancer; Authors - Yansong Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

DAN (Verified Customer) (08-19-2019)

the CHST4 antibody doesn't work as expected

• Applications: Western Blot,

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