Anticorps Polyclonal de lapin anti-CHT1

CHT1 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21848-1-PBS

Synonymes

SLC5A7, Hemicholinium-3-sensitive choline transporter, hCHT1, hCHT, CHT

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Galerie de données de validation

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  WB analysis of mouse brain using 21848-1-AP (same clone as 21848-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21848-1-AP (CHT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21848-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 21848-1-AP (same clone as 21848-1-PBS)

  IP result of anti-CHT1 (IP:21848-1-AP, 4ug; Detection:21848-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 21848-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21848-1-AP (same clone as 21848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21848-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21848-1-AP (same clone as 21848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21848-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 21848-1-AP (same clone as 21848-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CHT1 antibody (21848-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21848-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21848-1-PBS cible CHT1 dans les applications de WB, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHT1 Protéine recombinante Ag16136
• Nom complet: solute carrier family 5 (choline transporter), member 7
• Masse moléculaire calculée: 580 aa, 63 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC111525
• Symbole du gène: CHT1
• Identification du gène (NCBI): 60482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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