Anticorps Monoclonal anti-CIB1

CIB1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

bovin, Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D2C12

N° de cat : 67901-1-PBS

Synonymes

DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1

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Galerie de données de validation

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  WB analysis using 67901-1-Ig (same clone as 67901-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67901-1-Ig (same clone as 67901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67901-1-Ig (same clone as 67901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67901-1-Ig (same clone as 67901-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CIB1 Monoclonal antibody (67901-1-Ig, Clone:1D2C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67901-1-PBS cible CIB1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons bovin, Humain, rat, souris

• Réactivité: bovin, Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CIB1 Protéine recombinante Ag31174
• Nom complet: calcium and integrin binding 1 (calmyrin)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000846
• Symbole du gène: CIB1
• Identification du gène (NCBI): 10519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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