Anticorps Polyclonal de lapin anti-CILP2

CILP2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11813-1-PBS

Synonymes

CILP-2, Cartilage intermediate layer protein 2 C2, Cartilage intermediate layer protein 2 C1

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Galerie de données de validation

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  WB analysis using 11813-1-AP (same clone as 11813-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 11813-1-AP (CILP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CILP2 antibody (11813-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11813-1-PBS cible CILP2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CILP2 Protéine recombinante Ag2366
• Nom complet: cartilage intermediate layer protein 2
• Masse moléculaire calculée: 126 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC034926
• Symbole du gène: CILP2
• Identification du gène (NCBI): 148113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)

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