Anticorps Monoclonal anti-CIN85

CIN85 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3C12D2

N° de cat : 66571-1-Ig

Synonymes

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

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Galerie de données de validation

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  WB analysis using 66571-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of K-562 using 66571-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66571-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66571-1-Ig

  rat brain and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Daudi, cellules Jurkat, cellules K-562, cellules PC-12, cellules Raji, cellules Ramos, cellules THP-1, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66571-1-Ig cible CIN85 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CIN85 Protéine recombinante Ag2777
• Nom complet: SH3-domain kinase binding protein 1
• Masse moléculaire calculée: 665 aa, 73 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC015806
• Symbole du gène: SH3KBP1
• Identification du gène (NCBI): 30011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.