Anticorps Monoclonal anti-CIN85

CIN85 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C12D2

N° de cat : 66571-1-PBS

Synonymes

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

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Galerie de données de validation

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  WB analysis using 66571-1-Ig (same clone as 66571-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66571-1-Ig (same clone as 66571-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66571-1-Ig (same clone as 66571-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66571-1-Ig (same clone as 66571-1-PBS)

  rat brain and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66571-1-PBS cible CIN85 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CIN85 Protéine recombinante Ag2777
• Nom complet: SH3-domain kinase binding protein 1
• Masse moléculaire calculée: 665 aa, 73 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC015806
• Symbole du gène: SH3KBP1/CIN85
• Identification du gène (NCBI): 30011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

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