Anticorps Monoclonal anti-CISD1

CISD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D7E3

N° de cat : 68030-1-PBS

Synonymes

1D7E3, C10orf70, CDGSH iron sulfur domain 1, CDGSH iron-sulfur domain-containing protein 1, Cysteine transaminase CISD1

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Galerie de données de validation

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  WB analysis using 68030-1-Ig (same clone as 68030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68030-1-Ig (CISD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 68030-1-Ig (same clone as 68030-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CISD1 antibody (68030-1-Ig, Clone: 1D7E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68030-1-PBS cible CISD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CISD1 Protéine recombinante Ag8560
• Nom complet: CDGSH iron sulfur domain 1
• Masse moléculaire calculée: 108 aa, 12 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC007043
• Symbole du gène: CISD1
• Identification du gène (NCBI): 55847
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

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