Anticorps Polyclonal de lapin anti-CIT

CIT Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20033-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of A431 using 20033-1-AP (same clone as 20033-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  WB analysis using 20033-1-AP (same clone as 20033-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20033-1-AP (same clone as 20033-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20033-1-AP (same clone as 20033-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20033-1-PBS cible CIT dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: citron (rho-interacting, serine/threonine kinase 21)
• Masse moléculaire calculée: 231 kDa
• Poids moléculaire observé: 230 kDa, 177 kDa
• Numéro d’acquisition GenBank: NM_007174
• Symbole du gène: CIT
• Identification du gène (NCBI): 11113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.

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