Anticorps Polyclonal de lapin anti-CITED2

CITED2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15021-1-PBS

Synonymes

MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 15021-1-AP (same clone as 15021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15021-1-AP (CITED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

15021-1-PBS cible CITED2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CITED2 Protéine recombinante Ag7078
• Nom complet: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 36 kDa, 27 kDa
• Numéro d’acquisition GenBank: BC004377
• Symbole du gène: CITED2
• Identification du gène (NCBI): 10370
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].

{{ptg:RelatedPrimaryAntibodies}}