Anticorps Polyclonal de lapin anti-CKAP2

• Phare
• Validé par KD/KO

CKAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 25486-1-AP

Synonymes

Cytoskeleton-associated protein 2, LB1, se20-10, TMAP, Tumor- and microtubule-associated protein

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Galerie de données de validation

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  WB analysis of HeLa using 25486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25486-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25486-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 25486-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 25486-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25486-1-AP (CKAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25486-1-AP

  Immunofluorescent analysis of HeLa cells using 25486-1-AP (CKAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules PC-3, cellules SGC-7901
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

25486-1-AP cible CKAP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKAP2 Protéine recombinante Ag21901
• Nom complet: cytoskeleton associated protein 2
• Masse moléculaire calculée: 683 aa, 77 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC136332
• Symbole du gène: CKAP2
• Identification du gène (NCBI): 26586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein (TMAP), is implicated in the regulation of cell proliferation (PMID: 30119212). CKAP2 is a MAP expressed during mitosis that localizes to centrosomes and spindles before being targeted for degradation by the anaphase-promoting complex/cyclosome at mitotic exit (PMID: 38381793, 17339342).

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation; Authors - Lia Mara Gomes Paim
human	WB	Oncol Rep; CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.; Authors - Kuanyu Wang ; KD Validated
human	WB,IF	Cancers (Basel); Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer; Authors - Alexsandro dos Santos
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azriel (Verified Customer) (12-02-2019)

Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: HT-1080 human fibrosarcoma cell line

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