Anticorps Polyclonal de lapin anti-CKAP2

• Phare
• Validé par KD/KO

CKAP2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 25486-1-AP

Synonymes

CKAP2, CTCL tumor antigen se20 10, LB1, se20 10, TMAP

---

Galerie de données de validation

• 

  WB analysis of HeLa using 25486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25486-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25486-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 25486-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 25486-1-AP

  Immunofluorescent analysis of HeLa cells using 25486-1-AP (CKAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules PC-3, cellules SGC-7901
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

25486-1-AP cible CKAP2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKAP2 Protéine recombinante Ag21901
• Nom complet: cytoskeleton associated protein 2
• Masse moléculaire calculée: 683 aa, 77 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC136332
• Symbole du gène: CKAP2
• Identification du gène (NCBI): 26586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Oncol Rep; CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.; Authors - Kuanyu Wang ; KD Validated
human	WB,IF	Cancers (Basel); Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer; Authors - Alexsandro dos Santos

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Azriel (Verified Customer) (12-02-2019)

Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: HT-1080 human fibrosarcoma cell line